Overview

Our preclinical methods program develops and validates in vitro protocols for evaluating compound activity in biological systems. We focus on creating reproducible, well-documented methodologies that can be used consistently across studies.

All methods are designated Research Use Only (RUO) and are intended to support laboratory investigation rather than clinical application. Our goal is to provide the research community with reliable tools for preclinical longevity research.

Capabilities

Cell viability and cytotoxicity assays
Proliferation and senescence markers
Metabolic activity measurements (glucose uptake, mitochondrial function)
Gene expression analysis (qPCR)
Protein expression and secretion (ELISA, Western blot)
Protocol development and validation

Method Development Areas

Senescence Assays

Standardized protocols for measuring senescence-associated beta-galactosidase and SASP markers.

Metabolic Panels

Coordinated assay sets for evaluating effects on glucose metabolism and lipid handling.

Mitochondrial Function

Methods for assessing mitochondrial membrane potential, oxygen consumption, and ATP production.

Documentation Standards

Every method developed at LLRI is fully documented with detailed protocols, validated performance characteristics, and known limitations. We provide clear guidance on appropriate applications and include quality control criteria for each assay.

This documentation supports reproducibility—both in our own laboratory and when methods are adopted by collaborators and the broader research community.